Quick note:
1. A260/280 ratio measures the contaminants from the prep in the sample (guanidine salts which is typically used in RNA preps to lyse cells and inhibit RNases).
The ideal range is between 1.8 - 2.1.
2. A260/230 ratio measures the level of protein contamination in the sample.
The ideal range is above 1.5.
3. The Nanodrop readings are not accurate if the RNA yields are in less amount (20 ng/ul or below). However, we can further determine the integrity of the RNA samples by taking a look at the wavelength plot data on the Nanodrop, if there is a nice downslope at the 230 reading followed by the peak at 260 and then the curve goes down again at 280, then the RNA is okay.
Reference:
http://www.mobio.com/blog/2012/08/03/high-quality-rna-in-accurate-results-out-how-to-qc-your-rna/
1. A260/280 ratio measures the contaminants from the prep in the sample (guanidine salts which is typically used in RNA preps to lyse cells and inhibit RNases).
The ideal range is between 1.8 - 2.1.
2. A260/230 ratio measures the level of protein contamination in the sample.
The ideal range is above 1.5.
3. The Nanodrop readings are not accurate if the RNA yields are in less amount (20 ng/ul or below). However, we can further determine the integrity of the RNA samples by taking a look at the wavelength plot data on the Nanodrop, if there is a nice downslope at the 230 reading followed by the peak at 260 and then the curve goes down again at 280, then the RNA is okay.
Reference:
http://www.mobio.com/blog/2012/08/03/high-quality-rna-in-accurate-results-out-how-to-qc-your-rna/
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